9 resultados para Manifestações oculares
em SAPIENTIA - Universidade do Algarve - Portugal
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Dissertação mest., Psicologia, Universidade do Algarve, 2008
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Dissertação de mest., Psicologia da Educação, Universidade do Algarve, 2007
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Dissertação de mest., Psicologia Clínica e da Saúde, Faculdade de Ciências Humanas e Sociais, Univ. do Algarve, 2011
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O património rural mediterrânico encerra um quadro de manifestações passadas e presentes com uma riqueza específica e uma diversidade de significados. No quadro de uma ampla parceria dialogante, com uma estrutura técnica entusiasta, dinâmica e crente no desenvolvimento do território do espaço do Mediterrâneo e Baixo Guadiana, foram feitas trocas de experiências, discutidos conceitos e metodologias de intervenção e analisados impactos de onde resultou para cada território um conjunto de acções- piloto de carácter transnacional que consubstanciassem as temáticas do projecto Rural Med, onde se enquadra o presente estudo do Baixo Guadiana.
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Artigo de divulgação das particularidades etnográficas da festa natalícia na região do Algarve, que foi publicado na revista de Natal no matutino lisboeta «Diário de Notícias» no ano de 1985 .
Resumo:
Ocular pathologies are among the most debilitating medical conditions affecting all segments of the population. Traditional treatment options are often ineffective, and gene therapy has the potential to become an alternative approach for the treatment of several pathologies. Methacrylate polymers have been described as highly biocompatible and are successfully used in medical applications. Due to their cationic nature, these polymers can be used to form polyplexes with DNA for its delivery. This work aims to study the potential of PDMAEMA (poly(2-(N,N’-dimethylamino)ethyl methacrylate)) as a non viral gene delivery system to the retina. The first part of this work aimed to study the potential for gene delivery of a previously synthesized PDMAEMA polymer of high molecular weight (354kDa). In the second part, we synthesized by RAFT a PDMAEMA with a lower molecular weight (103.3kDa) and similarly, evaluated its ability to act as a gene delivery vehicle. PDMAEMA/DNA polyplexes were prepared at 5, 7.5, 10, 12.5 and 20 nitrogen/phosphorous (N/P) ratio for the 354kDa PDMAEMA and at 5 and 7.5 for the 103.3kDa PDMAEMA. Dynamic light scattering and zeta potential measurements confirmed the nanosize and positive charge of polyplexes for all ratios and for both polymers. Both high and low Mw PDMAEMA were able to efficiently complex and protect DNA from DNase I degradation. Their cytotoxicity was evaluated using a non-retinal cell line (HEK293) and a retinal pigment epithelium (RPE) cell line (D407). We have found that cytotoxicity of the free polymer is concentration and time dependent, as expected, and negligible for all the concentrations of the PDMAEMA-DNA polyplexes. Furthermore, for the concentrations to be used in vivo, the 354kDa PDMAEMA showed no signs of inflammation upon injection in the intravitreal space of C57BL/6 mice. The transfection efficiency, as evaluated by fluorescence microscopy and flow cytometry, showed that the D407 retinal cells were transfected by polyplexes of both high and low Mw PDMAEMA, but with varied efficiency, which was dependent on the N/P ratio. Althogether, these results suggest that PDMAEMA is a feasible candidate for non-viral gene delivery to the retina, and this work constitutes the basis of further studies to elucidate the bottleneck in transfection and further optimization of the material.
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Dissertação de mestrado, Gestão Cultural, Faculdade de Ciências Humanas e Sociais, Universidade do Algarve, 2013
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Dissertação de mestrado, Psicologia Clínica e da Saúde, Faculdade de Ciências Humanas e Sociais, Universidade do Algarve, 2015
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Dissertação de mestrado, Ciências Farmacêuticas, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015
